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Expression characteristics of the USP24 gene in the mouse testis during spermatogenesis / 中华男科学杂志
Zhonghua nankexue ; Zhonghua nankexue;(12): 963-968, 2017.
Article de Zh | WPRIM | ID: wpr-812849
Bibliothèque responsable: WPRO
ABSTRACT
Objective@#To investigate the expression characteristics of the USP24 gene in the mouse testis and its role in spermatogenesis.@*METHODS@#We examined the expression characteristics of USP24 in the testis tissues of wild-type mice at different postnatal weeks (PNW) and androgen receptor (AR)-knockout (ARKO) adult mice using real-time quantitative PCR and immunofluorescence, and detected the transcriptional activity of the USP24 promoter by dual-luciferase reporter gene assay.@*RESULTS@#The expression of the USP24 gene was low in the testis tissue of the wild-type mice at PNW 1, increased dramatically at PNW 3 and stayed at a similar level till PNW 8. The USP24 protein was located mainly in the cytoplasm of Sertoli and spermatogenic cells. Compared with the wild-type, the adult ARKO mice showed a decreased expression of USP24 localized in the posterior head and mid-piece of the mature sperm in the testis. Dual-luciferase reporter gene assay showed that the transcriptional activity of the USP24 promoter was increased after testosterone stimulation.@*CONCLUSIONS@#The increased expression of the USP24 gene was associated with the initiation of sexual development, and the USP24 protein was expressed in the mature sperm of the mice. USP24 is an AR-target gene, which may be involved in the regulation of spermatogenesis in mice.
Sujet(s)
Mots clés
Texte intégral: 1 Indice: WPRIM Sujet Principal: Cellules de Sertoli / Spermatogenèse / Spermatozoïdes / Testicule / Testostérone / Transcription génétique / Récepteurs aux androgènes / Régions promotrices (génétique) / Souris knockout / Ubiquitin thiolesterase Limites du sujet: Animals langue: Zh Texte intégral: Zhonghua nankexue Année: 2017 Type: Article
Texte intégral: 1 Indice: WPRIM Sujet Principal: Cellules de Sertoli / Spermatogenèse / Spermatozoïdes / Testicule / Testostérone / Transcription génétique / Récepteurs aux androgènes / Régions promotrices (génétique) / Souris knockout / Ubiquitin thiolesterase Limites du sujet: Animals langue: Zh Texte intégral: Zhonghua nankexue Année: 2017 Type: Article