Simultaneous Determination of 7 Constituents in Xiao ’er Jinqiao Granules by QAMS / 中国药房
China Pharmacy
; (12): 1179-1184, 2020.
Article
de Zh
| WPRIM
| ID: wpr-821603
Bibliothèque responsable:
WPRO
ABSTRACT
OBJECTIVE:To e stablish a method for simultaneous determination of 7 constituents in Xiao ’er jinqiao granules . METHODS:QAMS method was adopted. The determination was performed on Lubex Kromasil C 18 column with mobile phase consisted of acetonitrile- 0.1% phosphoric acid solution (gradient elution ). The detection wavelength was set at 326 nm,the flow rate was 1.0 mL/min. The column temperature was 40 ℃,and sample size was 10 µL. Using chlorogenic acid as the internal reference,the relative correction factors (RCF)of neochlorogenic acid ,cryptochlorogenic acid ,forsythiaside A ,isochlorogenic acid B ,isochlorogenic acid A and isochlorogenic acid C were calculated. The effects of different chromatogram system , chromatogram column ,the ratio of mobile phase ,flow rate and column temperature on RCF were investigaten. According to the two-point correction method combined with the relative retention time correction of the components to be tested ,the peak location was carried out. The contents of 7 components were determined by QAMS and SCM respectively and then compared. RESULTS: The linear range of neochlorogenic acid ,chlorogenic acid ,cryptochlorogenic acid ,forsythoside A ,isochlorogenic acid B ,isochlorogenic acid A ,isochlorogenic acid C were 9.27-92.70,37.36-373.60,13.02-130.20,7.15-71.50,4.56-45.60,6.32-63.20,14.69-146.90 µg/mL (r≥0.999 7). The limits of quantification were 1.38,1.41,1.40,1.99,1.10,1.17,1.10 ng,respectively;and the limits of detection were 0.41,0.42,0.42,0.60,0.33,0.35,0.33 ng,respectively. RSDs of precision ,repeatability and stability tests were all less than 2%;average recoveries were 98.28%-99.15% (RSD<2.0% , n=9). RCFs of neochlorogenic acid , (No.2013LZLY-K61) cryptochlorogenic acid ,forsythiaside A ,isochlorogenic acid B,isochlorogenic acid A and isochlorogenic acid C relative to xinyuliang@stu.cqmu.edu.cn chlorogenic acid were 0.995,1.007,0.580,1.243,1.252 and 1.247,respectively. RSDs of RCFs were all lower than 3% under different chromatogram conditions. Absolute value of relative error between the relative retention time of components to be tested predicted by two-point correction combined with relative retention time correction and measured value was less than 2%. The contents measured by QAMS were 2.790-3.416, 14.526-17.907,3.763-4.531,1.625-1.982,1.087-1.523,1.434-2.219,3.631-5.078 mg/g;the contents measured by SCM method were 2.811-3.438,14.512-17.893,3.739-4.508,1.656-2.012,1.108-1.544,1.460-2.245,3.597-5.045 mg/g;relative errors of the two methods were within ±2%. CONCLUSIONS :Two-point correction method combined with relative retention time correction can accurately locate the peaks of each constituent ;established QAMS method is simple ,rapid,accurate and reliable ,it can be used for simultaneous determination of 7 constituents in Xiao ’er jinqiao granules.
Texte intégral:
1
Indice:
WPRIM
Type d'étude:
Prognostic_studies
langue:
Zh
Texte intégral:
China Pharmacy
Année:
2020
Type:
Article