Disruption of OsRhoGDI2 by CRISPR/Cas9 technology leads to semi-dwarf in rice / 生物工程学报
Chinese Journal of Biotechnology
; (12): 707-715, 2020.
Article
Dans Zh
| WPRIM
| ID: wpr-826905
Responsable en Bibliothèque :
WPRO
ABSTRACT
OsRhoGDI2 was isolated as a putative partner of Rho protein family member OsRacD from rice panicles by yeast two-hybrid, but its function remains unknown. In order to identify the function of OsRhoGDI2, OsRhoGDI2 knockout mutants were created by CRISPR/Cas9 technology. The results showed that two different homozygous mutants were obtained in T0 generation, and eight kinds homozygous mutants were identified in T1 generation. Sequence analysis revealed that the base substitution or base deletion occurred near the editing targets of the gene in knockout rice, and it could be expected that the truncated OsRhoGDI2 proteins lacking the RhoGDI conserved domain would be generated. Phenotype analysis showed that the OsRhoGDI2 knockout rice plants were significantly lower than the control plants. Statistical analysis confirmed that the significant decrease of plant height was due to the shortening of the second and third internodes, suggesting that OsRhoGDI2 gene may be related with rice height control.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Oryza
/
Végétaux génétiquement modifiés
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Gènes de plante
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Inhibiteur bêta de la dissociation des nucléotides guanylés spécifique des protéines rho
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Systèmes CRISPR-Cas
/
Génétique
langue:
Zh
Texte intégral:
Chinese Journal of Biotechnology
Année:
2020
Type:
Article