Polymorphism of HLA-A, -B, -C, -DRB1 and -DQB1 Allele and Haplotype Frequency at High Resolution in Guangxi Zhuang Population / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1397-1405, 2020.
Article
Dans Zh
| WPRIM
| ID: wpr-827105
Responsable en Bibliothèque :
WPRO
ABSTRACT
OBJECTIVE@#To analyze the characteristics of allelic and haplotypic polymorphisms of human leukocyte antigens at HLA-A, -B, -C, DRB1 and DQB1 loci in Guangxi Zhuang population.@*METHODS@#Polymerase chain reaction-sequence based typing (PCR-SBT) was used to detect. The five loci (HLA-A, -B, -C, -DRB1, -DQB1) in 350 unrelated Zhuang ethnic individual from Guangxi region. Allelic and haplotypic frequencies were calculated by using Arlequin software 3.5.2.2. Phylogeny tree were constructed by using MEGA software 6.0, and SPSS software was used for principal component analysis.@*RESULTS@#Among the five loci in the population, only HLA-A and DRB1 loci were observed as departures from Hardy-Weinberg expectations. A total of 19 HLA-A, 42 HLA-B, 22 HLA-C, 25 HLA-DRB1 and 15 HLA-DQB1 alleles were found in 350 samples. The most highest frequent alleles were A*11 01(28.57%), B*46 01(14.00%), C*01 02(18.43%), DRB1*16 02 (15.71%)and DQB1*05 02 (35.00%) . The most common five loci haplotype was A*33 03-C*03 02-B*58 01-DRB1*03 01-DQB1*02 01(6.86%). The phylogenetic tree analysis showed that Guangxi Zhuang population had a relative close genetic relationship with southern Han Chinese populations.@*CONCLUSION@#This reaserch found that the HLA-A, B, C, DRB1 and DQB1 loci are highly polymorphic in Guangxi Zhuang population.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Phylogenèse
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Haplotypes
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Antigènes HLA-A
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Antigènes HLA-B
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Chine
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Allèles
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Chaines HLA-DRB1
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Fréquence d'allèle
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Génétique
Limites du sujet:
Humans
Pays comme sujet:
Asia
langue:
Zh
Texte intégral:
Journal of Experimental Hematology
Année:
2020
Type:
Article