Generation of a cold-adapted PRRSV with a nucleotide substitution in the ORF5 and numerous mutations in the hypervariable region of NSP2
Journal of Veterinary Science
; : e85-2020.
Article
de En
| WPRIM
| ID: wpr-833732
Bibliothèque responsable:
WPRO
ABSTRACT
A cold-adapted porcine reproductive and respiratory syndrome virus (CA-VR2332) was generated from the modified live virus strain VR2332. CA-VR2332 showed impaired growth when cultured at 37°C with numerous mutations ( S731F , E819D , G975E , and D1014N) in the hypervariable region of the NSP2, in which the mutation S731F might play a vital role in viral replication at 30°C. Conserved amino acid sequences of the GP5 protein suggests that CAVR2332 is a promising candidate for producing an effective vaccine against PRRSV infection.Further studies on replication and immunogenicity in vivo are required to evaluate the properties of CA-VR2332.
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WPRIM
langue:
En
Texte intégral:
Journal of Veterinary Science
Année:
2020
Type:
Article