Determination of hesperidin and liquiritin in Bailong Jieyu granule by high-performance liquid chromatography / 第二军医大学学报

ABSTRACT

Objective To determine the contents of the hesperidin and liquiritin in Bailong Jieyu granule with high-performance liquid chromatography (HPLC). Methods HPLC was performed with octadecylsilane chemically bonded silica as filler using the Agilent SB-ZOBAX C18 Column (250 mm×4.6 mm, 5 μm). Hesperidin was detected with the mobile phase consisting of acetonitrile and 0.1% of phosphoric acid solution (22:78), with column temperature being 30 °C, the detection wavelength being 283 nm, and the injection volume being 10 μL. Liquiritin was determined with acetonitrile-0.1% of phosphoric acid solution (18:82) as mobile phase, with column temperature being 30 °C, the detection wavelength being 276 nm, and the injection volume being 10 μL. Results Accuracy, repeatability and stability of HPLC for determination of hesperidin and liquiritin were in line with the relevant requirements. The linear relationship of hesperidin was good within the range of 35.64-178.20 μg/mL (r=0.999 8), with the average recovery rate being 99.30%, and relative standard deviation (RSD) being 1.0% (n=9). The good linear range of liquiritin was 13.55-216.80 μg/mL (r=0.999 9), with the average recovery rate being 98.60% and RSD being 1.5% (n=9). Conclusion HPLC method was fast, simple, stable and reliable, and it can be used for the quality control of Bailong Jieyu granule.