Puerarin inhibits the proliferation and ossification of fibroblasts in ankylosing spondylitis / 西安交通大学学报(医学版)

ABSTRACT

Objective: To explore the effect and underlying mechanism of puerarin on cell proliferation and ossification of fibroblasts in ankylosing spondylitis (AS). Methods: Fibroblasts were isolated from the hip capsule tissue of AS patients or femoral neck fractures. The cells were treated with puerarin at different concentrations (25, 50, 100, 150, and 200 mg/L). Cell proliferation was measured by CCK-8 method. Protein expressions of IL-6, TNF-α, OCN and OPN were measured by Western blot. ALP activity was detected and the mRNA expression of Col I was evaluated by qRT-PCR. Results: Puerarin of 150 and 200 mg/L significantly inhibited the proliferation of normal fibroblasts, while puerarin of 100, 150 and 200 mg/L significantly inhibited the proliferation of AS fibroblasts. Puerarin significantly inhibited protein expressions of pro-inflammatory cytokines IL-6 and TNF-α, decreased ALP activity, inhibited mRNA expression ofCol, and attenuated protein expressions of OCN and OPN. Conclusion: Puerarin inhibits cell proliferation and fibroblast ossification of normal and AS fibroblasts.