Construction of the knockdown rat model of programmed cell death 4 in vivo / 西安交通大学学报(医学版)

ABSTRACT

Objective: To construct the rat Pdcd4 knockdown model of programmed cell death 4 (Pdcd 4) in vivo for further study of the gene's functions. Methods: E3 rats were intranasally instilled with saline or plasmids during antigen induced pulmonary inflammation model induction. The rats were anaesthetized and killed on day 21. The lungs in each group were lavaged by instillation and withdrawal of 2 mL ice-cold PBS for three times through the tracheal route, and the bronchoalveolar lavage fluid (BALF) was collected. After centrifugation, the BALF cell pellet was used to isolate the total RNA. And the mRNA level of Pdcd4 was assessed by real-time quantitative PCR. Results: Pdcd4 expression decreased significantly in BALF cells at the mRNA level after Pdcd4 RNAi in vivo. Conclusion: Rat Pdcd4 knockdown model in vivo was constructed successfully, which can lay foundation for further studies on the functions and mechanism of Pdcd4 gene.