Screening of active components of Jitongning Tablet by liposome equilibrium dialysis and LC-MS analysis / 中草药

ABSTRACT

Objective: To explore the interaction between the effective components of Jitongning Tablet (JT) and liposome biomembrance, in order to predict in vivo absorption of the effective components. Methods: Liposome as the model of biomembrance, active components were screened by liposome equilibrium dialysis and LC-MS; And the effect of experiment conditions on the interaction of the liposome concentration, and the pH value of buffer were also investigated. Results: There were 11 compounds in JT with obvious interaction with liposome, including 4 flavonoids, 2 triterpenoid, 4 coumarins, and 1 phenylpropionic acid. The concentration of liposome and pH value of buffer remarkably affected the interaction between JT and liposome. Conclusion: The established method contributes to predicting the multiple components absorption in body, indicating the potentially active compounds and providing reference for further research of pharmacodynamic substance basis.