Isolation and identification of dehydrocorydaline and glaucine from Corydalis Rhizoma by using high-speed counter-current chromatography and UPLC-Q-TOF-MS/MS / 中草药
Zhongcaoyao
; Zhongcaoyao;(24): 4351-4356, 2016.
Article
de Zh
| WPRIM
| ID: wpr-853092
Bibliothèque responsable:
WPRO
ABSTRACT
Objective: To isolate dehydrocorydaline and glaucine by high-speed counter-current chromatography (HSCCC) from the extraction of Corydalis Rhizoma (CR). Methods: A mixture of chloroform-n-butanol-methanol-water (4:1:2:5) was used as the two phase solvent system both in forward and reversal direction, with a flow rate of 10.0 mL/min and a rotary speed of 800 r/min eluting for 30 min. The detection wavelength was 282 nm and injection volume was 50 mg. The purity of the target product was analyzed by HPLC-UV and the structure was identified by ultra performance liquid chromatography-tandem quadrupole time-of- flight mass spectrometry (UPLC-Q-TOF-MS/MS). Results: Under optimized conditions, 7.1 mg and 3.4 mg of two compounds were obtained and their yields were 81.43% and 91.11% respectively. Their purities were 98.9% and 94.3% detected by HPLC. dehydrocorydaline and glaucine were identifiled through HPLC, ultraviolet absorbance, and UPLC-Q-TOF-MS/MS. Conclusion: The result indicate that HSCCC is a powerful technique for the purification of dehydrocorydaline and glaucine from CR.
Texte intégral:
1
Indice:
WPRIM
Type d'étude:
Diagnostic_studies
langue:
Zh
Texte intégral:
Zhongcaoyao
Année:
2016
Type:
Article