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Isolation and identification of dehydrocorydaline and glaucine from Corydalis Rhizoma by using high-speed counter-current chromatography and UPLC-Q-TOF-MS/MS / 中草药
Zhongcaoyao ; Zhongcaoyao;(24): 4351-4356, 2016.
Article de Zh | WPRIM | ID: wpr-853092
Bibliothèque responsable: WPRO
ABSTRACT
Objective: To isolate dehydrocorydaline and glaucine by high-speed counter-current chromatography (HSCCC) from the extraction of Corydalis Rhizoma (CR). Methods: A mixture of chloroform-n-butanol-methanol-water (4:1:2:5) was used as the two phase solvent system both in forward and reversal direction, with a flow rate of 10.0 mL/min and a rotary speed of 800 r/min eluting for 30 min. The detection wavelength was 282 nm and injection volume was 50 mg. The purity of the target product was analyzed by HPLC-UV and the structure was identified by ultra performance liquid chromatography-tandem quadrupole time-of- flight mass spectrometry (UPLC-Q-TOF-MS/MS). Results: Under optimized conditions, 7.1 mg and 3.4 mg of two compounds were obtained and their yields were 81.43% and 91.11% respectively. Their purities were 98.9% and 94.3% detected by HPLC. dehydrocorydaline and glaucine were identifiled through HPLC, ultraviolet absorbance, and UPLC-Q-TOF-MS/MS. Conclusion: The result indicate that HSCCC is a powerful technique for the purification of dehydrocorydaline and glaucine from CR.
Mots clés
Texte intégral: 1 Indice: WPRIM Type d'étude: Diagnostic_studies langue: Zh Texte intégral: Zhongcaoyao Année: 2016 Type: Article
Texte intégral: 1 Indice: WPRIM Type d'étude: Diagnostic_studies langue: Zh Texte intégral: Zhongcaoyao Année: 2016 Type: Article