Comparison on progeny regeneration capacity among different mating patterns and optimization of cultivation condition in Swertia mileensis / 中草药

ABSTRACT

Objective: To establish and optimize the technique of tissue culture and rapid propagation, and compare the progeny regeneration capacity among different mating patterns in Swertia mileensis, meanwhile, to explore the reproductive assurance of the fragment populations. Methods: Sterilized seeds from different mating patterns were cultured on MS media for germination, which included self-breed, inbreeding, outbreeding, and nature pollinating, and counted the germination rate 60 d later. The study used the different source explants maintained on MS medium supplemented with different types and concentration of plant growth regulator combinations to explore its capacity of callus induction and adventitious shoots differentiation with orthogonal design. Results: There is no significant difference in the germination rates among all kinds of seed as touched above. The optimal medium for callus induction is MS + ZT 0.5 mg/L + 2,4-D 0.05 mg/L, the callus induction rates were 100%, 96.67%, 96.55%, and 96.29%; The optimal medium for adventitious shoots differentiation was MS + BA 2.0 mg/L + KT 0.1 mg/L + NAA 1.0 mg/L, and the differentiation rates all reached up to 100%; The optimal medium for rooting was 1/2 MS + NAA 1.0 mg/L, the rooting rate both were as high as 100% and there was no significant difference in the growth of plantlets. Conclusion: This study optimizes the cultivation conditions and provides an effective solution for protecting the wild resources and sprouts multiplication of S. mileensis. Meanwhile, from the plant physiological basis, it could be preliminarily predicted that the advantage of inbreeding would make up the fitness cost associated with it and the progeny regeneration capacity shows no significant difference between inbreeding and outbreeding.