Chemical constituents from Selaginella doederleinii and their bioactivities / 中草药

ABSTRACT

Objective: To study the chemical constituents from Selaginella doederleinii and their bioactivities. Methods: Column chromatography on silica gel, RP-C18, and Sephadex LH-20 columns was used to separate and purify the chemical constituents. The structures were elucidated by physicochemical properties and spectroscopic analyses. The inhibition of EtOAc fraction on the tumor cells was tested in vivo and in vitro. Results: Eleven compounds were obtained from EtOAc fraction and identified as flavonoids and phenolic acids. These compounds were asamentoflavone (1), robustafavone 4'-methyl ether (2), 5, 5″, 7, 7″, 4', 4‴-hexahydroxy-(2', 8″)-biflavone (3), 7, 4', 7″, 4‴-tetra-O-amentoflavone (4), 5, 5″, 7, 7″, 4', 4‴-hexahydroxy-(2', 6″)-biflavone (5), ginkgetin (6), isoginkgetin (7), quercetin-3-O-α-D-arabinfuranosi (8), nobiletin (9), gallic acid (10), and shikimic acid (11). Two alkaloids, berberine (12) and palmatine (13) were obtained from n-BuOH fraction. The bioassays indicated that EtOAc fraction could inhibit the growth of HeLa and HepG2 in vitro with IC50 values of 0.12 and 0.60 mg/mL, respectively. The tumor-inhibition rate in S180-bearing mice was 43%. Conclusion: Compound 5 is a new natural product, and compounds 3, 6-10, 12, and 13 are isolated from the plant for the first time.