Evaluation of d-galactose induced osteoporosis in male mice by micro CT and preventive effect of calcitriol / 中国药理学通报

ABSTRACT

Aim: To observe the iconography and biomechanical changes of femur in this D-galactose induced osteoporosis model in male mice, using Micro CT and other methods, and to observe the effect of calcitriol in this model. Methods: Thirty SPF male KM mice of 17 weeks old were randomly divided into control group, model group and calcitriol group, and the drug administration lasted for 12 weeks. At the end of the experiment, the mice were sacrificed and the bilateral femurs were taken for a bio-mechanical three-point bending test and a Micro CT scan. Results: Compared with control group, the Micro CT index of the femur in model group showed that Conn. D., BMD, BV/TV and Tb. N were significantly reduced (P < 0. 05), and SMI was raised significantly (P < 0. 05). Compared with model group, the BMD, BV/TV, Tb. N and Tb. Th in calcitriol group were significantly raised (P <0. 05), and SMI was significantly reduced (P < 0. 05). What's more, the break load, elastic load, maximum load and stiffness coefficient in calcitriol group significantly increased (P < 0. 05). Conclusions: D-galactose can induce a visible iconography changes of osteoporosis in male mice. Calcitriol can significantly improve the micro structure of trabecular bone and increase the bio-mechanical properties of femur in D-galactose male mice.