Detection of Four Aflatoxins in Gelatin Drugs by Immunoaffinity Column Clean-up and HPLC-MS/MS / 中国药学杂志

ABSTRACT

OBJECTIVE: To develop an immunoaffinity column clean-up and high performance liquid chromatography coupled with triple quadrupole mass spectrometry(HPLC-MS/MS) method to determine aflatoxins in gelatin drugs. METHODS: The analysis was performed by an HPLC-MS/MS system with X-Brigde-C18(3.0 mm×50 mm, 3.5 μm)column. Multiple-reaction monitoring (MRM) was performed to identify and quantify aflatoxin B1, B2, G1 and G2, which were extracted from Asini Coril Colla, Cervil Cornus Colla, and Testudinis Carapacis Colla with 60% methanol solution. RESULTS: Linear calibration curves were obtained with r≥0.997 9. The precision of the method was showed by RSDs (n=6) ranging from 1.2% to 4.1%. The recoveries were determined at three concentration levels and ranged from 77.3% to 94.6%. The ranges of LOQs were from 0.5 to 0.8 μg·L-1 and the RSDs (n=9) of intra-day precision and inter-day precision were from 1.1% to 2.9% and from 1.5% to 2.8%, respectively. CONCLUSION: The method is specific, simple and rapid to detect aflatoxins in gelatin drugs.