Content Determination of Four Triterpenes in Caragana sinica by HPLC and α-glucosidase Activity Assay in Vitro / 中国药学杂志
Chinese Pharmaceutical Journal
; (24): 488-493, 2017.
Article
Dans Zh
| WPRIM
| ID: wpr-858778
Responsable en Bibliothèque :
WPRO
ABSTRACT
OBJECTIVE:
To establish HPLC methods for the determination of betulinic acid, oleanolic acid, ursolic acid, and lupeol in Caragana sinica roots, and investigate the α-glucosidase activity of the four compounds in vitro.METHODS:
An Agilent Eclipse XDB-C18 column (4.6 mm × 250 mm, 5 μm) was applied with methanol-water-phosphoric acid (85150.1) as the mobile phase at a flow rate of 0.6 mL·min-1 to separate betulinic acid, ursolic acid, and oleanolic acid. An Inert Sustain C18 column (4.6 mm × 150 mm, 5 μm) was applied with acetonitrile-water (7030) as the mobile phase at a flow rate of 0.8 mL·min-1 to separate lupeol.RESULTS:
Good linearities were achieved for betulinic acid, oleanolic acid, ursolic acid, and lupeol within the range of 0.56-5.58, 0.42-4.20, 0.19-1.92, and 6.10-61.00 μg, respectively. The average recoveries were 99.79%, 98.51%, 98.05%, and 99.47%, and the RSDs were 1.53%, 1.74%, 1.78%, and 1.94%, respectively.CONCLUSION:
The developed methods are accurate and can be used for the quality control of Caragana sinica. In addition to betulinic acid, the other three compounds show good α-glucosidase inhibitory activity and are expected to be developed as new hypoglycemic drugs.
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WPRIM
langue:
Zh
Texte intégral:
Chinese Pharmaceutical Journal
Année:
2017
Type:
Article