Performance of the Real-Q EBV Quantification Kit for Epstein-Barr Virus DNA Quantification in Whole Blood
Annals of Laboratory Medicine
; : 147-150, 2017.
Article
de En
| WPRIM
| ID: wpr-8648
Bibliothèque responsable:
WPRO
ABSTRACT
There has been increasing interest in standardized and quantitative Epstein-Barr virus (EBV) DNA testing for the management of EBV disease. We evaluated the performance of the Real-Q EBV Quantification Kit (BioSewoom, Korea) in whole blood (WB). Nucleic acid extraction and real-time PCR were performed by using the MagNA Pure 96 (Roche Diagnostics, Germany) and 7500 Fast real-time PCR system (Applied Biosystems, USA), respectively. Assay sensitivity, linearity, and conversion factor were determined by using the World Health Organization international standard diluted in EBV-negative WB. We used 81 WB clinical specimens to compare performance of the Real-Q EBV Quantification Kit and artus EBV RG PCR Kit (Qiagen, Germany). The limit of detection (LOD) and limit of quantification (LOQ) for the Real-Q kit were 453 and 750 IU/mL, respectively. The conversion factor from EBV genomic copies to IU was 0.62. The linear range of the assay was from 750 to 10⁶ IU/mL. Viral load values measured with the Real-Q assay were on average 0.54 log₁₀ copies/mL higher than those measured with the artus assay. The Real-Q assay offered good analytical performance for EBV DNA quantification in WB.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Trousses de réactifs pour diagnostic
/
ADN viral
/
Herpèsvirus humain de type 4
/
Infections à virus Epstein-Barr
/
Limite de détection
/
Réaction de polymérisation en chaine en temps réel
Limites du sujet:
Humans
langue:
En
Texte intégral:
Annals of Laboratory Medicine
Année:
2017
Type:
Article