Establishment, evaluation, and determination of saliva glucose concentration by ion chromatography / 中华内分泌外科杂志
Chinese Journal of Endocrine Surgery
; (6): 61-65, 2021.
Article
Dans Zh
| WPRIM
| ID: wpr-882712
Responsable en Bibliothèque :
WPRO
ABSTRACT
Objective:
To establish an analytical method for measuring the concentration of glucose in saliva by ion chromatography.Methods:
The proteins in saliva were removed by thermal denaturation method, CarboPac PA20 (3×30 mm) was used as a protective column and CarboPac PA20 (3×150 mm) was used as an analytical column for ion chromatography analysis. Gradient elution was carried out with A ultra-pure water, B 250 mmol/L NaOH solution and C 500 mmol/L NaAc solution. Pulsed ampere detector was used for detection.Results:
This method had a good linear relationship in the range of 0.04 to 0.12 mg/L, with a linear relation coefficient of 0.9967. The detection limit of glucose was 2 μg/L, the mean value of the relative standard deviation (RSD) of the repeatability measurement was 0.75%, and the average spike recovery was 103.07%.Conclusion:
This method is simple, sensitive, accurate and stable, and can be used for the determination of glucose concentration in saliva.
Texte intégral:
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Indice:
WPRIM
langue:
Zh
Texte intégral:
Chinese Journal of Endocrine Surgery
Année:
2021
Type:
Article