Research progress on in vitro models of cardiomyocyte injury / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 3257-3269, 2021.
Article
Dans Zh
| WPRIM
| ID: wpr-887975
Responsable en Bibliothèque :
WPRO
ABSTRACT
Cardiovascular diseases seriously endanger human health and life. The accompanying myocardial injury has been a focus of attention in society. Chinese medicine,serving as a natural and precious reservoir for the research and development of new drugs,is advantageous in resisting myocardial injury due to its multi-component,multi-pathway,and multi-target characteristics. In recent years,with the extensive application of culture method for isolated cardiomyocytes,a cost-effective,controllable in vitro model of cardiomyocyte injury with uniform samples is becoming a key tool for mechanism research on cardiomyocyte injury and drug development.A good in vitro model can reduce experimental and manpower cost,and also accurately stimulate clinical changes to reveal the mechanism. Therefore,the selection and establishment of in vitro model are crucial for the in-depth research. This study summarized the modeling principles,evaluation indicators,and application of more than ten models reflecting different clinical conditions,such as injuries induced by hypoxia-reoxygenation,hypertrophy,oxidative stress,inflammation,internal environmental disturbance,and toxicity. Furthermore,we analyzed advantages and technical difficulties,aiming to provide a reference for in-depth research on myocardial injury mechanism and drug development.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Hypoxie cellulaire
/
Apoptose
/
Stress oxydatif
/
Myocytes cardiaques
/
Myocarde
Type d'étude:
Prognostic_studies
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2021
Type:
Article