Expression and Localization of Aquaporins in Benign Prostate Hyperplasia and Prostate Cancer / 전남의대학술지
Chonnam Medical Journal
; : 174-178, 2012.
Article
Dans En
| WPRIM
| ID: wpr-90300
Responsable en Bibliothèque :
WPRO
ABSTRACT
The aquaporin (AQP) families of water channels are intrinsic membrane proteins that facilitate selective water and small solute movement across the plasma membrane. The purposes of this study were to determine the expression and localization of AQPs in benign prostatic hyperplasia and prostate cancer. Prostatic tissue was collected from patients with benign prostatic hyperplasia or prostate cancer by transurethral resection of the prostate. The expression and cellular localization of the AQPs were determined in the human prostate by Western blot and immunohistochemistry. AQP1, 3, and 9 were expressed in the human prostate. Western blot analysis revealed bands at 28-36 kDa for the AQP1, 3, and 9 proteins. Of these proteins, AQP3 and 9 were expressed in the epithelium. Immunolabeling showed that AQP1 was mainly expressed in the capillaries and venules of the prostate, AQP9 was expressed in the cytoplasm of the epithelium, and AQP3 was mainly associated with the plasma membrane of the prostatic epithelium. Only AQP3 expression was localized in the cell membrane, and expressed AQP3 was translocated to the cytoplasm in prostate cancer. The epithelium in the human prostate expresses AQP3 and 9 proteins, and the capillaries and venules of the prostate express AQP1. Characterizing or modifying the expression of AQP3 may lead to an understanding of the role of the AQPs in human prostatic disease.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Prostate
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Maladies de la prostate
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Hyperplasie de la prostate
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Tumeurs de la prostate
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Veinules
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Vaisseaux capillaires
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Immunohistochimie
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Protéines
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Membrane cellulaire
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Technique de Western
Limites du sujet:
Humans
langue:
En
Texte intégral:
Chonnam Medical Journal
Année:
2012
Type:
Article