Research progress of POCT of nucleic acid based on CRISPR/Cas technology / 中华检验医学杂志

ABSTRACT

In view of given that the current development trend of nucleic acid molecular detection technology is gradually shifting from clinical laboratory to field detection, there is an urgent need to establish a new nucleic acid diagnostic tool with high sensitivity, high specificity, great efficiency and convenience portability to meet the needs of clinical point-of-care testing(POCT). The detection method based on clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein (Cas) is a promising new nucleic acid detection method. In this method, the Cas effector protein would recognize highly specific nucleic acid sequences, and it can be used for POCT in combination with a highly sensitive small biosensor. This method can successfully achieves the high-efficiency and convenient detection characteristics while satisfying high sensitivity and high specificity. This article reviews the developments progress in the field of miniaturized analytical sensors related to CRISPR/Cas technology in recent years, and discusses the research prospects and challenges of related POCT detection.