Cyclic Stretch Promotes Migration of MC3T3-E1 Cells via Increasing Runx2 Expression / 医用生物力学
Journal of Medical Biomechanics
; (6): E162-E168, 2022.
Article
de Zh
| WPRIM
| ID: wpr-920685
Bibliothèque responsable:
WPRO
ABSTRACT
Objective To investigate the effects of cyclic stretch on migration of MC3T3-E1 cells and its related mechanism. Methods The strain loading system was used to stretch MC3T3-E1 cells cultured in vitro with 15% amplitude, to simulate the mechanical condition in vivo. The wound healing assay was used to detect the migration of MC3T3-E1 cells. Western blotting was used to test Runx2 expression. RNA interfering was used to decrease Runx2 expression. Results Cyclic mechanical stretch with 15% amplitude, 1.25 Hz frequency and lasting for 24 hours could promote the migration of MC3T3-E1 cells and increase the expression level of Runx2. Runx2 interference inhibited the migration of MC3T3-E1 cells in static culture condition. Interference with Runx2 expression in MC3T3-E1 cells could partially reduce the positive effect of cyclic mechanical stretch on cell migration. Conclusions Cyclic stretch can promote the migration of MC3T3-E1 cells, and Runx2 may play an important role in this process. This study provides experimental basis for finding innovative clinical treatment method to promote fracture healing.
Texte intégral:
1
Indice:
WPRIM
langue:
Zh
Texte intégral:
Journal of Medical Biomechanics
Année:
2022
Type:
Article