Role of miR-145-5p Targeting ARK5 in Regulating the Proliferation and Apoptosis of Human Epithelial Ovarian Cancer Cells / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 669-676, 2021.
Article
de Zh
| WPRIM
| ID: wpr-921525
Bibliothèque responsable:
WPRO
ABSTRACT
Objective To explore the effect of miR-145-5p on the proliferation and apoptosis of human ovarian cancer cells and the possible molecular mechanisms involved.Methods Real-time quantitative PCR was performed to detect the expression of miR-145-5p in ovarian epithelial cells and ovarian cancer cells.CCK-8 and flow cytometry were used to detect the effects of miR-145-5p overexpression on the proliferation and apoptosis of ovarian cancer cells.TargetScan was employed to predict the target genes of miR-145-5p.Western blotting,dual luciferase reporter assay and rescue experiment were employed to predict and verify the underlying molecular mechanism of miR-145-5p function.Results The expression of miR-145-5p in ovarian cancer cells was significantly lower than that in normal ovarian epithelial cells(
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Tumeurs de l'ovaire
/
Régulation de l'expression des gènes tumoraux
/
Apoptose
/
MicroARN
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Lignée cellulaire tumorale
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Prolifération cellulaire
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Carcinome épithélial de l'ovaire
Limites du sujet:
Female
/
Humans
langue:
Zh
Texte intégral:
Acta Academiae Medicinae Sinicae
Année:
2021
Type:
Article