UHPLC-Q-TOF/MS analysis of chemical constituents in compound Jinqiancao granules / 药学实践杂志
Journal of Pharmaceutical Practice
; (6): 146-151, 2022.
Article
de Zh
| WPRIM
| ID: wpr-923028
Bibliothèque responsable:
WPRO
ABSTRACT
Objective To qualitatively analyze the main chemical components in compound Jinqiancao granules by ultra high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF/MS). Methods XBridge BEH C18 column (2.1 mm×100 mm, 2.5 µm) was used for chromatographic separation. The mobile phase was composed of 0.1% formic acid water and 0.1% formic acid-acetonitrile, gradient elution, and the flow rate was 0.4 ml/min. Mass spectrometry was characterized by Quadrupole time-of-flight mass spectrometry (Q-TOF/MS) and positive ion mode scanning. Results Under the optimized LC/MS condition, 47 components in compound Jinqiancao granules were identified. The isomers were distinguished by software calculation. The source of medicinal materials was assigned. Conclusion A rapid and efficient analytical method was established for the identification of chemical components in compound Jinqiancao granules by UHPLC-Q-TOF/MS.
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Indice:
WPRIM
langue:
Zh
Texte intégral:
Journal of Pharmaceutical Practice
Année:
2022
Type:
Article