The Effect of Extracellular Collagen on Synthesis of Extracellular Matrix in a 3-Dimensional Culture of Intervertebral Disc Cells / 대한척추외과학회지
Journal of Korean Society of Spine Surgery
; : 70-77, 2002.
Article
Dans Ko
| WPRIM
| ID: wpr-92550
Responsable en Bibliothèque :
WPRO
ABSTRACT
STUDY DESIGN:
In-vitro experimental study.OBJECTIVES:
To determine the proteoglycan synthesis of the rabbit nucleus pulposus cells in various concentration of extracellular collagen type I and II under the stimulation of TGF-beta1. SUMMARY OF LITERATURE REVIEW Therapeutic effect of growth factor and gene therapy can be altered by composition of extracellular matrix. However, the effect of extracellular collagen types I and II on synthetic activity of intervertebral disc cells is not thoroughly studied before. MATERIALS ANDMETHODS:
The nucleus pulposus cells were isolated and cultured from 10 skeletally mature rabbits. Cultures were trypsinized and incorporated into alginate beads with different concentration of extracellular collagen type I and II (0.5%, 1.0% and 1.5%). Those cultures with TGF-beta1 (10 ng/ml) served stimulated condition of matrix synthesis. Newly synthesized proteoglycans were assessed by 35 S-sulfate incorporation using chromatography on Sephadex G-25 in PD-10 columns. Scintillation count was normalized with DNA content by Hoechst dye method.RESULTS:
In basal condition, difference in proteoglycan synthesis in given concentration of extracellular collagen type I and II were statistically insignificant. In stimulated condition with TGF-beta1, difference in proteoglycan synthesis in given concentration of extracellular collagen type I and II was also statistically insignificant. However, cultures in stimulated condition with TGF-beta1 showed increased amount of newly synthesized proteoglycans compared to those of basal condition regardless of the concentration of extracellular collagen type I and II (p < 0.05).CONCLUSION:
Anabolic response of rabbit nucleus pulposus cells is relatively insensitive to extracellular matrix composition, which facilitates application of gene therapy in various conditions of disc degeneration.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Protéoglycanes
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ADN
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Trypsine
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Thérapie génétique
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Chromatographie
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Collagène
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Collagène de type I
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Matrice extracellulaire
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Facteur de croissance transformant bêta-1
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Dégénérescence de disque intervertébral
Limites du sujet:
Animals
langue:
Ko
Texte intégral:
Journal of Korean Society of Spine Surgery
Année:
2002
Type:
Article