A new method for isolating gas vesicles from Microcystis for ultrasound contrast / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1589-1601, 2022.
Article
Dans Zh
| WPRIM
| ID: wpr-927803
Responsable en Bibliothèque :
WPRO
ABSTRACT
Gas vesicles are a unique class of gas-filled protein nanostructures which are commonly found in cyanobacteria and Halobacterium. The gas vesicles may scatter sound waves and generate harmonic signals, which enabled them to have the potential to become a novel ultrasound contrast agent. However, the current hypertonic cracking method for isolating gas vesicles contains tedious operational procedures and is of low yield, thus not suitable for large-scale application. To overcome these technical challenges, we developed a rapid and efficient method for isolating gas vesicles from Microcystis. The new H2O2-based method increased the yield by three times and shortened the operation time from 24 hours to 7 hours. The H2O2 method is not only suitable for isolation of gas vesicles from laboratory-cultured Microcystis, but also suitable for colonial Microcystis covered with gelatinous sheath. The gas vesicles isolated by H2O2 method showed good performance in ultrasound contrast imaging. In conclusion, this new method shows great potential for large-scale application due to its high efficiency and wide adaptability, and provides technical support for developing gas vesicles into a biosynthetic ultrasonic contrast agent.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Protéines
/
Cyanobactéries
/
Produits de contraste
/
Microcystis
/
Peroxyde d'hydrogène
langue:
Zh
Texte intégral:
Chinese Journal of Biotechnology
Année:
2022
Type:
Article