The signal sequence of type II porcine reproductive and respiratory syndrome virus glycoprotein 3 is sufficient for endoplasmic reticulum retention
Journal of Veterinary Science
; : 307-313, 2013.
Article
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| ID: wpr-92900
Responsable en Bibliothèque :
WPRO
ABSTRACT
The glycoprotein 3 (GP3) of type II porcine reproductive and respiratory syndrome virus has the characteristic domains of a membrane protein. However, this protein has been reported to be retained in the endoplasmic reticulum (ER) rather than transported to the plasma membrane of the cell. In this study, we performed confocal laser scanning microscopy analysis of variants of GP3 and foundthat the signal sequence of the GP3 led to confinement of GP3 in the ER, while the functional ortransmembrane domain did not affect its localization. Based on these results, we concludedthat the signal sequence of GP3 contains the ER retention signal, which might play an important role in assembly of viral proteins.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Plasmides
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Signaux de triage des protéines
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Lignée cellulaire
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Membrane cellulaire
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Protéines de l'enveloppe virale
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Microscopie confocale
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Virus du syndrome respiratoire et reproducteur porcin
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Analyse de séquence de protéine
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Réticulum endoplasmique
Limites du sujet:
Animals
langue:
En
Texte intégral:
Journal of Veterinary Science
Année:
2013
Type:
Article