Identification and analysis of 2 Corynebacterium diphtheria strains in Guangdong Province / 中华预防医学杂志
Chinese Journal of Preventive Medicine
; (12): 427-432, 2022.
Article
Dans Zh
| WPRIM
| ID: wpr-935303
Responsable en Bibliothèque :
WPRO
ABSTRACT
Objective:
To identify and analyze two strains of C. diphtheriae in Guangdong Province by combining whole genome sequencing with traditional detection methods.Methods:
The C. diphtheriae was isolated from Guangzhou in 2010 and Zhuhai in 2020 respectively. Isolates were identified by API Coryne strips and MALDI-TOF-MS. Genomic DNA was sequenced by using Illumina. The assembly was performed for each strain using CLC software. J Species WS online tool was used for average nucleoside homology identification, then narKGHIJ and tox gene were detected by NCBI online analysis tool BLSATN. MEGA-X was used to build a wgSNP phylogenetic tree.Results:
GD-Guangzhou-2010 was Belfanti and GD-Zuhai-2020 was Gravis. ANIb between GD-Guangzhou-2010 and C. belfantii was 99.61%. ANI between GD-Zhuhai-2020 and C. diphtheriae was 97.64%. BLASTN results showed that the nitrate reduction gene narKGHIJ and tox gene of GD-Guangzhou-2010 was negative, while GD-Zhuhai-2020 nitrate reduction gene narKGHIJ was positive. There were two obvious clades in wgSNP phylogenetic tree. The first clades included all Mitis and Gravis types strains as well as GD-Zhuhai-2020. The second clades contained all isolates of C.belfantii, C.diphtheriae subsp. lausannense and GD-guangzhou-2010.Conclusion:
Two non-toxic C. diphtheriae strains are successfully isolated and identified. The phylogenetic tree suggests that GD-Guangzhou-2010 and GD-Zhuhai-2020 are located in two different evolutionary branches.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Phylogenèse
/
Chine
/
Corynebacterium
/
Corynebacterium diphtheriae
/
Diphtérie
/
Nitrates
Type d'étude:
Diagnostic_studies
Limites du sujet:
Humans
Pays comme sujet:
Asia
langue:
Zh
Texte intégral:
Chinese Journal of Preventive Medicine
Année:
2022
Type:
Article