Agkistrodon halys venom antitumor component-I inhibits vasculogenic mimicry in triple-negative breast cancer cells in vitro by down-regulating MMP2 / 南方医科大学学报
Journal of Southern Medical University
; (12): 438-442, 2022.
Article
Dans Zh
| WPRIM
| ID: wpr-936335
Responsable en Bibliothèque :
WPRO
ABSTRACT
OBJECTIVE@#To investigate the inhibitory effect of agkistrodon halys venom antitumor component-I (AHVAC-I) on vasculogenic mimicry (VM) formation in triple-negative breast cancer MDA-MB-231 cells and explore its possible mechanism.@*METHODS@#CCK8 assay was used to determine the optimal concentration of AHVAC-I for cell treatment based on its halfinhibitory concentration (IC50). MDA-MB-231 cells were treated with different concentrations of AHVAC-I or 5-Fu, and the changes in vasomimetic capacity of the cells were examined using Matrigel assay. The expression levels of matrix metalloproteinase-2 (MMP2) and MMP9 in the treated cells were detected using quantitative PCR and Western blotting.@*RESULTS@#Compared with the control treatment with culture medium, treatment with 5, 10 and 20 μg/mL AHVAC-I significantly reduced vasomimetic ability of MDA-MB-231 cells in a dose-dependent manner (P < 0.01). MMP2 supplementation obviously restored the vasomimetic ability of the cells inhibited by AHVAC-I.@*CONCLUSION@#AHVAC-I inhibits VM formation in triplenegative breast cancer cells in vitro by down-regulating MMP2 production.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Venins
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Agkistrodon
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Matrix metalloproteinase 2
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Lignée cellulaire tumorale
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Tumeurs du sein triple-négatives
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Espérance de vie en bonne santé
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Néovascularisation pathologique
Limites du sujet:
Animals
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Humans
langue:
Zh
Texte intégral:
Journal of Southern Medical University
Année:
2022
Type:
Article