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The scheme for validation of clinical metagenomics sequencing assay / 中华检验医学杂志
Article de Zh | WPRIM | ID: wpr-958598
Bibliothèque responsable: WPRO
ABSTRACT
Clinical metagenomic next-generation sequencing (mNGS) entails unbiased shotgun sequencing of all microbial and host nucleic acids present in a clinical sample. By analyzing the microbiota diversity, taxonomic, and phylogenetic relationships of clinical specimens, metagenomics related analysis provides an opportunity to investigate substantial biological significance of different microbes. According to the published paper, most studies on mNGS mainly focused on the clinical impact evaluation. However, the studies focused on the analytical performance validation of mNGS before clinical application were rare. Here, a scheme, included intended use, method establishment, assay validation and standard operating protocol, for the laboratory validation of clinical metagenomics sequencing assay was provided by summarizing experiences of clinical laboratory department of Peking Union Medical College Hospital protocol and relevant research. In this scheme, we discussed important topics of mNGS laboratory validation as below: specimen type and pathogen list, bioinformatics pipeline setup, dry lab standard preparation and performance validation, mNGS workflow setup, background nucleotide acid evaluation, wet lab standard preparation and performance validation.
Mots clés
Texte intégral: 1 Indice: WPRIM langue: Zh Texte intégral: Chinese Journal of Laboratory Medicine Année: 2022 Type: Article
Texte intégral: 1 Indice: WPRIM langue: Zh Texte intégral: Chinese Journal of Laboratory Medicine Année: 2022 Type: Article