Genetic and expression analysis of the KCNRG gene in hepatocellular carcinomas
Experimental & Molecular Medicine
; : 247-255, 2006.
Article
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| ID: wpr-96566
Responsable en Bibliothèque :
WPRO
ABSTRACT
The potassium channels are ubiquitous multisubunit membrane proteins, and potassium-dependent alterations in the membrane potential play an important role in the proliferation of many types of cells. This study analyzed the mutation, allelic loss and expression patterns of the KCNRG gene in 77 HCCs in order to determine if the KCNRG gene, which encodes the potassium channel regulating protein, is involved in the tumorigenesis of hepatocellular carcinoma (HCC). One KCNRG missense mutation, CGT->CAT (Arg->His) was found at codon 92 within the T1 domain. Hep3B hepatoma cells were transfected with the wild- or mutant-KCNRG to determine the effect of this mutation in KCNRG. Interestingly, the suppressive cell growth activity of the mutant-type KCNRG was significantly lower than that of the wild-type KCNRG. In addition, allelic loss was detected in 17 out of 64 (26.5%) informative HCC cases, and all were hepatitis B virus (HBV)-positive. Moreover, the allelic loss was closely related to an intrahepatic metastasis (P=0.0247), higher grade (P=0.0078) and clinical stage (P=0.0071). Expression analysis revealed 22 tumor tissues to have a loss of expression of the KCNRG transcript. These results suggest that genetic alterations and the expression of KCNRG might play an important role in the development and/or progression of a subset of HCCs.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Analyse de mutations d'ADN
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Transfection
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Canaux potassiques
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Régulation de l'expression des gènes tumoraux
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Technique de Western
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Carcinome hépatocellulaire
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Polymorphisme de conformation simple brin
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Perte d'hétérozygotie
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RT-PCR
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Lignée cellulaire tumorale
Limites du sujet:
Adult
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Aged
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Aged80
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Female
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Humans
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Male
langue:
En
Texte intégral:
Experimental & Molecular Medicine
Année:
2006
Type:
Article