Advances in heterologous expression, structural elucidation and molecular modification of pullulanase / 生物工程学报
Chinese Journal of Biotechnology
; (12): 4432-4448, 2022.
Article
de Zh
| WPRIM
| ID: wpr-970325
Bibliothèque responsable:
WPRO
ABSTRACT
Starch is composed of glucose units linked by α-1, 4-glucoside bond and α-1, 6-glucoside bond. It is the main component of foods and the primary raw material for starch processing industry. Pullulanase can effectively hydrolyze the α-1, 6-glucoside bond in starch molecules. Combined with other starch processing enzymes, it can effectively improve the starch utilization rate. Therefore, it has been widely used in the starch processing industry. This paper summarized the screening of pullulanase-producing strain and its encoding genes. In addition, the effects of expression elements and fermentation conditions on the production of pullulanase were summarized. Moreover, the progress in crystal structure elucidation and molecular modification of pullulanase was discussed. Lastly, future perspectives on pullulanase research were proposed.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Amidon
/
Glycosidases
langue:
Zh
Texte intégral:
Chinese Journal of Biotechnology
Année:
2022
Type:
Article