RHD Gene Analysis of A Blood Donor with Del Phenotype / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 843-849, 2023.
Article
Dans Zh
| WPRIM
| ID: wpr-982139
Responsable en Bibliothèque :
WPRO
ABSTRACT
OBJECTIVE@#To analyze the RHD genotype of a blood donor with Del phenotype in Yunnan.@*METHODS@#Rh serological phenotype was identified. RHD gene was detected by PCR-SSP typing, and its 10 exons were sequenced. Exon 9 was amplified for sequencing and analysis. RHD zygosity was detected.@*RESULTS@#The Rh phenotype of this specimen was CcDelee. Genomic DNA exhibited a 1 003 bp deletion spanning from intron 8, across exon 9 into intron 9. The deletion breakpoints occurred between two 7-bp short tandem repeat sequences. There was no variation in the sequences of the remaining exons. The Rh hybridization box test showed that there was one RHD negative allele.@*CONCLUSION@#This specimen is Del type caused by deletion of RHD exon 9.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Phénotype
/
Système Rhésus
/
Donneurs de sang
/
Chine
/
Exons
/
Allèles
/
Génotype
Limites du sujet:
Humans
Pays comme sujet:
Asia
langue:
Zh
Texte intégral:
Journal of Experimental Hematology
Année:
2023
Type:
Article