Lysohexaenetides A and B, linear lipopeptides from Lysobacter sp. DSM 3655 identified by heterologous expression in Streptomyces / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 454-458, 2023.
Article
de En
| WPRIM
| ID: wpr-982716
Bibliothèque responsable:
WPRO
ABSTRACT
Lysobacter harbors a plethora of cryptic biosynthetic gene clusters (BGCs), albeit only a limited number have been analyzed to date. In this study, we described the activation of a cryptic polyketide synthase (PKS)/nonribosomal peptide synthetase (NRPS) gene cluster (lsh) in Lysobacter sp. DSM 3655 through promoter engineering and heterologous expression in Streptomyces sp. S001. As a result of this methodology, we were able to isolate two novel linear lipopeptides, lysohexaenetides A (1) and B (2), from the recombinant strain S001-lsh. Furthermore, we proposed the biosynthetic pathway for lysohexaenetides and identified LshA as another example of entirely iterative bacterial PKSs. This study highlights the potential of heterologous expression systems in uncovering cryptic biosynthetic pathways in Lysobacter genomes, particularly in the absence of genetic manipulation tools.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Streptomyces
/
Famille multigénique
/
Polyketide synthases
/
Lysobacter
/
Lipopeptides
langue:
En
Texte intégral:
Chinese Journal of Natural Medicines (English Ed.)
Année:
2023
Type:
Article