DNA polymorphism detection of Papaver somniferum L using fluorescent amplified fragment length polymorphism / 法医学杂志
Journal of Forensic Medicine
; (6): 262-267, 2008.
Article
Dans Zh
| WPRIM
| ID: wpr-983391
Responsable en Bibliothèque :
WPRO
ABSTRACT
OBJECTIVE@#To detect DNA polymorphism of Papaver somniferum L using fluorescent Amplified Fragment Length Polymorphism.@*METHODS@#Genomic DNA was isolated using the AxyPrep DNA Kit, double-digested by two restrictional endonucleases (Eco RI and Mse I) and ligated to oligonucleotide adapters. After Pre-amplification and selective amplification, the DNA fragments were separated by capillary electrophoresis using the CEQ8000 DNA Fragment Analyzer.@*RESULTS@#More than 20 fragments of highly polymorphic products were obtained by 8 pairs of primer from 64 selective amplifying primer pairs.@*CONCLUSION@#The fluorescent AFLP technique can be used to detect the DNA polymorphism of Papaver somniferum.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Papaver
/
Polymorphisme génétique
/
ADN des plantes
/
Génétique légale
/
Analyse de polymorphisme de longueur de fragments amplifiés
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Colorants fluorescents
langue:
Zh
Texte intégral:
Journal of Forensic Medicine
Année:
2008
Type:
Article