Isolating sperm cells by laser capture microdissection technique from mixture sample / 法医学杂志
J. forensic med
; Fa yi xue za zhi;(6): 33-42, 2011.
Article
de Zh
| WPRIM
| ID: wpr-983622
Bibliothèque responsable:
WPRO
ABSTRACT
OBJECTIVE@#To assess the application value of laser capture microdissection (LCM) technique for isolating a small number of sperm cells from mixture sample.@*METHODS@#Mixture samples were prepared with sperm cells and vaginal epithelia at different concentrations. Both LCM technique and the differential lysis method were employed to obtain sperm cells from the mixture samples, and DNA was extracted by magnetic beads method. STR genotyping was determined using Identifiler kit.@*RESULTS@#The successful STR genotype rate of sperm cells isolated from mixture samples with LCM technique was 92.86% (13/14). The rate of differential lysis method was 7.14% (1/14). The successful rates between the two methods were statistically different (P < 0.05).@*CONCLUSION@#LCM technique can effectively exclude the interference of female cell component and isolate a small number of sperm cells to obtain a single male STR genotyping. LCM technique is obviously better than the differential lysis method.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Spermatozoïdes
/
Coloration et marquage
/
Vagin
/
ADN
/
Séparation cellulaire
/
Réaction de polymérisation en chaîne
/
Profilage d'ADN
/
Séquences répétées en tandem
/
Cellules épithéliales
/
Microdissection au laser
Limites du sujet:
Female
/
Humans
/
Male
langue:
Zh
Texte intégral:
Fa yi xue za zhi
/
J. forensic med
Année:
2011
Type:
Article