Degenerate oligonucleotide primed-PCR technology establishment and its sensitivity test analysis / 法医学杂志
Journal of Forensic Medicine
; (6): 41-43, 2012.
Article
Dans Zh
| WPRIM
| ID: wpr-983711
Responsable en Bibliothèque :
WPRO
ABSTRACT
OBJECTIVE@#To establish a whole genome amplification testing system based on degenerate oligonucleotide primed-PCR (DOP-PCR) and to explore its reliability and sensitivity.@*METHODS@#DOP-PCR amplified production was detected by fluorescent labeled multiplex STR amplification and capillary electrophoresis detection system to determine reliability and sensitivity of DOP-PCR system.@*RESULTS@#DOP-PCR system was successfully established and the detection sensitivity reached 5 cells (30 pg) by pretreatment of DOP-PCR and then detection of STR genotyping.@*CONCLUSION@#The system established in this study is reliable and more testing sensitive for forensic trace evidence.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Oligonucléotides
/
ADN
/
Amplification de gène
/
Réaction de polymérisation en chaîne
/
Reproductibilité des résultats
/
Profilage d'ADN
/
Sensibilité et spécificité
/
Amorces ADN
/
Électrophorèse capillaire
/
Séquences répétées en tandem
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Journal of Forensic Medicine
Année:
2012
Type:
Article