Wound Age Estimation by Neutrophil Migration Distance / 法医学杂志
Journal of Forensic Medicine
; (6): 166-170, 2019.
Article
Dans En
| WPRIM
| ID: wpr-984993
Responsable en Bibliothèque :
WPRO
ABSTRACT
Objective To explore the application of neutrophil migration distance for wound age estimation of skeletal muscles in rats, and to provide methodological basis for follow-up study in future. Methods The skeletal muscle contusion model was established in rats, and the control group and the 2, 4, 6 h post-traumatic groups were set. The law of response of neutrophils that participated in the inflammation after injury was detected by immunohistochemical staining, and the relationship between neutrophil migration distance and injury time was detected by TissueFAXS PLUS software. Results The skeletal muscle was obviously infiltrated with neutrophils 2-6 h after injury. The positive rate of neutrophil was (28.75±0.94)% at 2 h post-traumatic, and reached the peak (45.50±3.63)% at 4 h post-traumatic, then decreased to (31.92±1.56)% at 6 h post-traumatic. The neutrophil migration distances increased with the progress of inflammation, and reached (124.80±12.32) μm, (229.03±21.45) μm and (335.04±16.75) μm at 2 h, 4 h and 6 h, respectively. Conclusion There is a relationship of neutrophil infiltrated number and migration distance and wound age within the 2-6 h after skeletal muscle injury, which could be used for the inference of skeletal muscle wound age.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Facteurs temps
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Études de suivi
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Rat Sprague-Dawley
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Muscles squelettiques
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Contusions
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Infiltration par les neutrophiles
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Granulocytes neutrophiles
Limites du sujet:
Animals
langue:
En
Texte intégral:
Journal of Forensic Medicine
Année:
2019
Type:
Article