Pathogenic characterization of Klebsiella pneumoniae resistant to carbapenems and polymyxin / 中华预防医学杂志
Chinese Journal of Preventive Medicine
; (12): 877-884, 2023.
Article
Dans Zh
| WPRIM
| ID: wpr-985490
Responsable en Bibliothèque :
WPRO
ABSTRACT
Objective: Analysis and investigation of pathogenic characteristics of polymyxin-and carbapenem-resistant Klebsiella pneumoniae (PR-CRKP). Methods: A total of 23 PR-CRKP strains isolated from clinical specimens from the General Hospital of Southern Theater Command from March 2019 to July 2021 were retrospectively collected, Whole-genome sequencing was performed on 23 PR-CRKP strains, resistance genes were identified by comparison of the CARD and the ResFinder database, high-resolution typing of PR-CRKP strains was analyzed by core genomic multilocus sequencing (cgMLST) and single nucleotide polymorphism (SNP); polymyxin resistance genes were determined by PCR and sequencing. Results: All PR-CRKP strains were KPC-2 producing ST11 types. cgMLST results showed that the evolutionary distance between the PR-CRKP strains and Klebsiella pneumoniae in mainland China was 66.44 on average, which is more closely related than foreign strains; the 23 PR-CRKP strains were divided into 3 main subclusters based on SNP phylogenetic trees, with some aggregation among Clade 2-1 in the isolation department and date. The two-component negative regulatory gene mgrB has seven mutation types including point mutations, different insertion fragments and different insertion positions. Conclusion: The close affinity of PR-CRKP strains indicate the possibility of nosocomial clonal transmission and the need to strengthen surveillance of PR-CRKP strains to prevent epidemic transmission of PR-CRKP.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Phylogenèse
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Bêta-Lactamases
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Tests de sensibilité microbienne
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Carbapénèmes
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Études rétrospectives
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Polymyxines
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Typage par séquençage multilocus
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Klebsiella pneumoniae
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Antibactériens
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Chinese Journal of Preventive Medicine
Année:
2023
Type:
Article