Turn down - turn up: a simple and low-cost protocol for preparing platelet-rich plasma
Clinics
; 74: e1132, 2019. tab, graf
Article
em En
| LILACS
| ID: biblio-1019702
Biblioteca responsável:
BR1.1
ABSTRACT
OBJECTIVE:
To describe and analyze a new protocol for the extraction of platelet-rich plasma (PRP) for use in clinical practice and compare this technique with methods that have been previously described in the medical literature.METHODS:
We extracted PRP from 20 volunteers using four different protocols (single spin at 1600 ×g, single spin at 600 ×g, double spin at 300 and 700 ×g, and double spin at 600 and 900 ×g). In another group of 12 individuals, we extracted PRP with our new technique (named 'turn down-turn up') consisting of a double spin (200 ×g and 1600 ×g) closed system using standard laboratory equipment (including an ordinary benchtop centrifuge), where the blood remained in the same tube during all processes, reducing the risk of contamination. Platelet counts adjusted to baseline values were compared using analysis of covariance (ANCOVA).RESULTS:
Using the four previously described protocols (mentioned above), we obtained concentrations of platelets that were 1.15-, 2.07-, 2.18-, and 3.19-fold greater than the baseline concentration, respectively. With the turn down-turn up technique, we obtained a platelet count that was 4.17-fold (95% confidence interval (CI) 3.09 to 5.25) greater than the baseline platelet count (p=0.063 compared with the double spin at 600 and 900 ×g method). The total cost of the disposable materials used in the extraction process was less than US$10.00 per individual.CONCLUSION:
In the present study, we described a simple and safe method for obtaining PRP using low-cost devices.Palavras-chave
Texto completo:
1
Índice:
LILACS
Assunto principal:
Centrifugação
/
Técnicas de Laboratório Clínico
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Plasma Rico em Plaquetas
Tipo de estudo:
Diagnostic_studies
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Evaluation_studies
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Guideline
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Health_economic_evaluation
Limite:
Adult
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Humans
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Male
Idioma:
En
Revista:
Clinics
Assunto da revista:
MEDICINA
Ano de publicação:
2019
Tipo de documento:
Article