Biopolymer Xanthan: A New Adjuvant for DNA Vaccines
Braz. arch. biol. technol
; 63: e20190090, 2020. graf
Article
em En
| LILACS
| ID: biblio-1132173
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract DNA vaccines have been evaluated as an option to prevent several diseases. In this study, the capacity of the xanthan biopolymer to improve the DNA vaccines immune response, administered intramuscularly, was evaluated. The experimental vaccines consisted of genes encoding fragments of the proteins LigA and LigB of Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni strain Fiocruz L1-130. The humoral immune response was evaluated by indirect ELISA. Cytokine expression levels were determined by RT-qPCR. Compared to the control group, the IgG antibody levels of animals immunized with pTARGET/ligAni and pTARGET/ligBrep plasmids associated with xanthan biopolymer were significantly higher than the control group. Additionally, there was a significant increase in IL-17 expression in animals vaccinated with pTARGET/ligBrep and xanthan.
Palavras-chave
Texto completo:
1
Índice:
LILACS
Assunto principal:
Polissacarídeos Bacterianos
/
DNA Recombinante
/
Adjuvantes Imunológicos
/
Xanthomonas campestris
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Vacinas de DNA
Limite:
Animals
Idioma:
En
Revista:
Braz. arch. biol. technol
Assunto da revista:
BIOLOGIA
Ano de publicação:
2020
Tipo de documento:
Article