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Two-Step Purification of L-Asparaginase from Acrylaway® L
Tundisi, Louise Lacalendola; Coêlho, Diego de Freitas; Faria, Alessandra Valéria de Sousa; Pessoa Junior, Adalberto; Tambourgi, Elias Basile; Nascimento, Laura de Oliveira; Silveira, Edgar; Mazzola, Priscila Gava.
Afiliação
  • Tundisi, Louise Lacalendola; University of Campinas. Institute of Biology. Graduate Program in Biosciences and Technology of Bioactive Products. Campinas. BR
  • Coêlho, Diego de Freitas; University of Campinas. Chemical Engineering School. Campinas. BR
  • Faria, Alessandra Valéria de Sousa; University of Campinas. Institute of Biology. Department of Biochemistry and Tissue Biology. BR
  • Pessoa Junior, Adalberto; University of São Paulo. Pharmaceutical Sciences School. São Paulo. BR
  • Tambourgi, Elias Basile; University of Campinas. Chemical Engineering School. Campinas. BR
  • Nascimento, Laura de Oliveira; University of Campinas. Pharmaceutical Sciences School. Campinas. BR
  • Silveira, Edgar; Federal University of Uberlândia. Genetics and Biochemistry Institute. Uberlândia. BR
  • Mazzola, Priscila Gava; University of Campinas. Pharmaceutical Sciences School. Campinas. BR
Braz. J. Pharm. Sci. (Online) ; 58: e191042, 2022. tab, graf
Article em En | LILACS | ID: biblio-1394057
Biblioteca responsável: BR40.1
Localização: BR40.1
ABSTRACT
Abstract L-Asparaginase (L-ASNase) is a biopharmaceutical used for acute lymphoblastic leukaemia (ALL) treatment, dramatically increasing the patients' chance of cure. However, its production and distribution in developing countries were disrupted because of its low profitability, which caused great concern among patients. This study evaluates the feasibility of combining fractional precipitation and aqueous two-phase systems (ATPS) to purify L-ASNase from a low-grade product, commercially known as Acrylaway® L. The ATPS purification results were not particularly expressive compared to the two-step purification process composed of ethanol precipitation and gel filtration, which was able to recover the target molecule with a purification factor over 5 fold. Thus, we studied a purification process capable of manufacturing pharmaceutical grade L-ASNase from a commercially available low-grade raw material; however, improvements regarding its throughput must be achieved, and high purity is the first step to apply it as a new biopharmaceutical product. The proposed process could pose as a short-time solution to mitigate its shortage while a cost-effective production plant is being developed.
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Texto completo: 1 Índice: LILACS Assunto principal: Asparaginase / Precipitação Fracionada / Antineoplásicos Idioma: En Revista: Braz. J. Pharm. Sci. (Online) Assunto da revista: Farmacologia / Terapˆutica / Toxicologia Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Índice: LILACS Assunto principal: Asparaginase / Precipitação Fracionada / Antineoplásicos Idioma: En Revista: Braz. J. Pharm. Sci. (Online) Assunto da revista: Farmacologia / Terapˆutica / Toxicologia Ano de publicação: 2022 Tipo de documento: Article