Serodiagnosis of visceral and cutaneous leishmaniasis in human and canine populations living in Indigenous Reserves in the Brazilian Amazon Region
Rev. Soc. Bras. Med. Trop
; 50(1): 61-66, Jan.-Feb. 2017. tab, graf
Article
em En
| LILACS
| ID: biblio-842819
Biblioteca responsável:
BR1.1
ABSTRACT
ABSTRACT INTRODUCTION:
Leishmaniasis is endemic to the Northern, Northeastern, Central-Western, and Southeastern regions of Brazil. We aimed to assess the epidemiological situation of leishmaniasis in humans and dogs in indigenous villages located in the States of Mato Grosso and Tocantins using a serological survey conducted in May 2011.METHODS:
Serum samples were collected from 470 humans and 327 dogs living in villages of the Urubu Branco and Tapirapé Karajá indigenous reserves. The samples were analyzed for the presence of Leishmania spp. antibodies using the indirect fluorescent antibody test (IFAT), enzyme-linked immunosorbent assay (ELISA) with a crude antigen (CA) and soluble antigen (SA), and Dual Path Platform (DPP®) immunoassay for canine visceral leishmaniasis.RESULTS:
Of 470 human samples tested, two (0.4%) were positive using IFAT. Among 327 dog samples tested, 28 (8.6%) were positive using ELISA CA, five (1.5%) using ELISA SA, two (0.6%) using IFAT, and none using DPP® immunoassay with Leishmania infantum chagasi antigen. When Leishmania amazonensis antigen was used, 20 (6.1%) samples were positive using ELISA CA and four (1.2%) using IFAT.CONCLUSIONS:
There was a low prevalence of infection in the region, and significant differences among the main serological methods used for the diagnosis of leishmaniasis. These findings indicated that the detection of Leishmania spp. requires further study and improvement.Palavras-chave
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Índice:
LILACS
Assunto principal:
Anticorpos Antiprotozoários
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Leishmaniose Cutânea
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Leishmania infantum
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Doenças do Cão
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Leishmaniose Visceral
Tipo de estudo:
Prevalence_studies
/
Risk_factors_studies
Limite:
Animals
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Female
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Humans
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Male
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Rev. Soc. Bras. Med. Trop
Assunto da revista:
MEDICINA TROPICAL
Ano de publicação:
2017
Tipo de documento:
Article