Perconditioning combined with postconditioning on kidney ischemia and reperfusion
Acta cir. bras
; 32(8): 599-606, Aug. 2017. tab, graf
Article
em En
| LILACS
| ID: biblio-886224
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract Purpose:
To evaluate if combination of perconditioning and postconditioning provides improved renal protection compared to perconditioning alone in a model of renal reperfusion injury.Methods:
Thirty rats were assigned into 6 groups normality; sham; ischemia and reperfusion; postconditioning; perconditioning; perconditioning + postconditioning. Animals were subjected to right nephrectomy and left renal ischemia for 30 minutes. Postconditioning consisted of 3 cycles of 5 min renal perfusion followed by 5 min of renal ischemia after major ischemic period. Perconditioning consisted of 3 cycles of 5 min hindlimb ischemia followed by 5 min of hindlimb perfusion contemporaneously to renal major ischemic period. After 24 hours, kidney was harvested and blood collected to measure urea and creatinine.Results:
Perconditioning obtained better values for creatinine and urea level than only postconditioning (p<0.01); performing both techniques contemporaneously had no increased results (p>0.05). Regarding tissue structure, perconditioning was the only technique to protect the glomerulus and tubules (p<0.05), while postconditioning protected only the glomerulus (p<0.05). Combination of both techniques shows no effect on glomerulus or tubules (p>0.05).Conclusions:
Perconditioning had promising results on ischemia and reperfusion induced kidney injury, enhanced kidney function and protected glomerulus and tubules. There was no additive protection when postconditioning and perconditioning were combined.Palavras-chave
Texto completo:
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Índice:
LILACS
Assunto principal:
Traumatismo por Reperfusão
/
Precondicionamento Isquêmico
/
Pós-Condicionamento Isquêmico
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Isquemia
/
Rim
Tipo de estudo:
Evaluation_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Acta cir. bras
Assunto da revista:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2017
Tipo de documento:
Article