Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis
Rev. Soc. Bras. Med. Trop
; 50(3): 365-373, May-June 2017. tab, graf
Article
em En
| LILACS
| ID: biblio-896971
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract INTRODUCTION:
The coral snake Micrurus surinamensis, which is widely distributed throughout Amazonia, has a neurotoxic venom. It is important to characterize the biological and molecular properties of this venom in order to develop effective antitoxins.METHODS:
Toxins from the venom of M. surinamensis were analyzed by two-dimensional polyacrylamide gel electrophoresis and their neurotoxic effects in vivo were evaluated. RESULTS ANDCONCLUSIONS:
Most proteins in the venom had masses < 14kDa, low phospholipase A2 activity, and no proteolytic activity. The toxins inhibited the coagulation cascade. The venom had neurotoxic effects in mice, with a median lethal dose upon intravenous administration of 700 µg/kg. Immunogenic studies revealed abundant cross-reactivity of antielapidic serum with 14kDa toxins and limited cross-reactivity with toxins < 10kDa. These results indicate that antielapidic serum against M. surinamensis venom has weak potency (0.35mg/ml) in mice.Palavras-chave
Texto completo:
1
Índice:
LILACS
Assunto principal:
Elapidae
/
Venenos Elapídicos
/
Fosfolipases A2
Limite:
Animals
País/Região como assunto:
America do sul
/
Caribe ingles
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Suriname
Idioma:
En
Revista:
Rev. Soc. Bras. Med. Trop
Assunto da revista:
MEDICINA TROPICAL
Ano de publicação:
2017
Tipo de documento:
Article