Effects of Er-Miao-San extracts on TNF-alpha-indueed MMP-1 expression in human dermal fibroblasts
Biol. Res
; 48: 1-8, 2015. graf
Article
em En
| LILACS
| ID: biblio-950773
Biblioteca responsável:
CL1.1
ABSTRACT
BACKGROUND:
Various health benefits have been attributed to Er-Miao-San (EMS), a traditional Chinese herbal formulation that contains equal amounts of cortex phellodendri (Phellodendron amurense Ruprecht) and rhizoma atractylodis (Atractylodes lancea D.C). However, its effect on the anti-inflammatory activity in human dermal fibroblasts (HDFs) and the mechanism underlying this effect are unknown.RESULTS:
This study investigated the effects of EMS on TNF-α-induced MMP-1 expression in HDFs. Our data show that EMS inhibited TNF-α-induced MMP-1 expression in a concentration-dependent manner. Interestingly, EMS maintained IkB content without inhibiting the phosphorylation of MAPKs, which are well-established upstream kinases of NF-kB. Moreover, EMS reduced the level of nuclear p65 protein in HDFs. Luciferase assay revealed that EMS inhibits the transcriptional activity of NF-kBbystabilizing IkB. Our results show that EMS exerts its anti-inflammatory effect by inhibiting NF-kB-regulated genes such as IL-1ß and IL-8. Moreover, EMS effectively inhibited TNF-α-induced expression of MMP-1 via the NF-kBpathway.CONCLUSIONS:
Taken together, our data suggest that EMS could potentially be used as an anti-inflammatory and anti-aging treatment.Palavras-chave
Texto completo:
1
Índice:
LILACS
Assunto principal:
Envelhecimento
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Medicamentos de Ervas Chinesas
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Extratos Vegetais
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Derme
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Metaloproteinase 1 da Matriz
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Fibroblastos
Limite:
Humans
Idioma:
En
Revista:
Biol. Res
Assunto da revista:
BIOLOGIA
Ano de publicação:
2015
Tipo de documento:
Article