Your browser doesn't support javascript.
loading
Inactivation of phoPQ genes attenuates Salmonella Gallinarum biovar Gallinarum to susceptible chickens
Alves, Lucas Bocchini Rodrigues; Freitas Neto, Oliveiro Caetano de; Batista, Diego Felipe Alves; Barbosa, Fernanda de Oliveira; Rubio, Marcela da Silva; Souza, Andrei Itajahy Secundo de; Almeida, Adriana Maria de; Barrow, Paul Andrew; Berchieri Junior, Angelo.
  • Alves, Lucas Bocchini Rodrigues; São Paulo State University. School of Agricultural and Veterinary Sciences. Post Graduate Program in Agricultural and Livestock Microbiology. Jaboticabal. BR
  • Freitas Neto, Oliveiro Caetano de; Federal University of Paraiba. Department of Veterinary Sciences. Areia. BR
  • Batista, Diego Felipe Alves; São Paulo State University. School of Agricultural and Veterinary Sciences. Post Graduate Program in Agricultural and Livestock Microbiology. Jaboticabal. BR
  • Barbosa, Fernanda de Oliveira; São Paulo State University. School of Agricultural and Veterinary Sciences. Post Graduate Program in Agricultural and Livestock Microbiology. Jaboticabal. BR
  • Rubio, Marcela da Silva; São Paulo State University. School of Agricultural and Veterinary Sciences. Post Graduate Program in Agricultural and Livestock Microbiology. Jaboticabal. BR
  • Souza, Andrei Itajahy Secundo de; São Paulo State University. School of Agricultural and Veterinary Sciences. Post Graduate Program in Agricultural and Livestock Microbiology. Jaboticabal. BR
  • Almeida, Adriana Maria de; São Paulo State University. School of Agricultural and Veterinary Sciences. Post Graduate Program in Agricultural and Livestock Microbiology. Jaboticabal. BR
  • Barrow, Paul Andrew; University of Nottingham. School of Veterinary Medicine and Science. Leicestershire. GB
  • Berchieri Junior, Angelo; São Paulo State University. School of Agricultural and Veterinary Sciences. Post Graduate Program in Agricultural and Livestock Microbiology. Jaboticabal. BR
Braz. j. microbiol ; 49(3): 601-606, July-Sept. 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-951806
ABSTRACT
Abstract Salmonella Gallinarum is a host-restrict pathogen that causes fowl typhoid, a severe systemic disease that is one of the major concerns to the poultry industry worldwide. When infecting the bird, SG makes use of evasion mechanisms to survive and to replicate within macrophages. In this context, phoPQ genes encode a two-component regulatory system (PhoPQ) that regulates virulence genes responsible for adaptation of Salmonella spp. to antimicrobial factors such as low pH, antimicrobial peptides and deprivation of bivalent cations. The role of the mentioned genes to SG remains to be investigated. In the present study a phoPQ-depleted SG strain (SG ΔphoPQ) was constructed and its virulence assessed in twenty-day-old laying hens susceptible to fowl typhoid. SG ΔphoPQ did cause neither clinical signs nor mortality in birds orally challenged, being non-pathogenic. Furthermore, this strain was not recovered from livers or spleens. On the other hand, chickens challenged subcutaneously with the mutant strain had discreet to moderate pathological changes and also low bacterial counts in liver and spleen tissues. These findings show that SG ΔphoPQ is attenuated to susceptible chickens and suggest that these genes are important during chicken infection by SG.
Assuntos


Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Doenças das Aves Domésticas / Salmonelose Animal / Proteínas de Bactérias / Salmonella enterica / Inativação Gênica Limite: Animais Idioma: Inglês Revista: Braz. j. microbiol Assunto da revista: Microbiologia Ano de publicação: 2018 Tipo de documento: Artigo País de afiliação: Brasil / Reino Unido Instituição/País de afiliação: Federal University of Paraiba/BR / São Paulo State University/BR / University of Nottingham/GB

Similares

MEDLINE

...
LILACS

LIS


Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Doenças das Aves Domésticas / Salmonelose Animal / Proteínas de Bactérias / Salmonella enterica / Inativação Gênica Limite: Animais Idioma: Inglês Revista: Braz. j. microbiol Assunto da revista: Microbiologia Ano de publicação: 2018 Tipo de documento: Artigo País de afiliação: Brasil / Reino Unido Instituição/País de afiliação: Federal University of Paraiba/BR / São Paulo State University/BR / University of Nottingham/GB