Identification and purification of a specific and immunogenic antigen of the laminated layer of the hydatid cyst and production of an antigen-specific monoclonal antibody
INTJVR-International Journal of Veterinary Research. 2010; 4 (4): 237-243
em En
| IMEMR
| ID: emr-143692
Biblioteca responsável:
EMRO
ABSTRACT
Cystic echinococcosis [CE] is an infection caused by the larval stage of Echinococcus granulosus. This is widely distributed through Iran, where a variety of animals act as intermediate host. The immunogenic antigens [Ag] of different compartments of the hydatid cyst have been already determined. One of these compartments is the laminated layer [LL]. We have extracted a protein with the MW of 24 kDa from a lysate prepared from the LL and produced a monoclonal antibody [mAb] against this protein. Five mAb named P[3]F[6s], P[2]Hp[4s], P[1], A[6s], P[1],C[3s], and P[1],F[7s] have been produced. The isotype analysis showed that P[3]F[6s] is IgG[1], and the rest are IgM. P[3]F[6s] was purified from the ascitic fluid of mice injected with P[3]F[6s] hybridoma intraperitoneally. Western blot and LLISA analysis showed that this mAb could recognize the purified 24 kDa prepared from lysate of LL. Since a 24 kDa protein has been shown to be an immunogenic Ag, this protein can be used as a candidate for the development of diagnostic tests and vaccine strategies. For these aims, P[3]F[6s] can be used for the purification of this 24 kDa protein
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Índice:
IMEMR
Assunto principal:
Anticorpos Monoclonais
/
Formação de Anticorpos
/
Antígenos de Protozoários
Idioma:
En
Revista:
Int. J. Vet. Res.
Ano de publicação:
2010