Purification and characterization of immunoglobulin G from Kala-Azar patients

ABSTRACT

The immunoglobulin G [lgG] of kala-azar patients was purified by ammonium sulfate precipitation and ion-exchange chromatography on DEAE-cellulose column. The purified IgG was found to be homogeneous as judge by polyacrylamide disc gel electrophoresis on 7.5% gel and SDS polyacrylamide gel electrophoresis. The molecular weight of IgG was estimated to be 158,489. The precipitation reaction was observed when the purified IgG was mixed with the serum of kala-azar patient [i.e., antigen]. Maximum precipitation was observed when IgG-antigen reaction at pH 7.0 and at 37°C. The specificity of the purified IgG was also confirmed from other serological tests such as haemagglutination and double diffusion tests