An alternative method for in vitro production of HIV-1-specific antibodies
Braz. j. med. biol. res
; 24(8): 797-9, 1991. tab
Article
em En
| LILACS
| ID: lil-102066
Biblioteca responsável:
BR1.1
RESUMO
We describe the detection of HIV-1-specific antibody secretion by a 24-h culture of peripheral blood mononuclear cells (PBMC) stimulated with disrupted inactivated whole virus absorved onto commercial ELISA microwell plates. The method showed high sensitivity and specificity and was capable of accurately detecting HIV-1 infection early after birth (9 of the 19 patients were < 3 years old) because maternal antibodies do not iterfere by giving false-postive results. No false-positive results were obtained with PBMC from 24 HIV-1-seronegative asymptomatic individuals and no false-negative results were obteined for 10 seropostive adult patients (16-46 years pf age). This rapid, relatively low cost, simple, highly sensitive and specific assay can be extremely useful for the early diagnosis of pediatric AIDS cases
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Índice:
LILACS
Assunto principal:
Técnicas In Vitro
/
Leucócitos Mononucleares
/
Anticorpos Anti-HIV
/
HIV-1
/
Soropositividade para HIV
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Adolescent
/
Adult
/
Child
/
Child, preschool
/
Humans
/
Infant
Idioma:
En
Revista:
Braz. j. med. biol. res
Assunto da revista:
BIOLOGIA
/
MEDICINA
Ano de publicação:
1991
Tipo de documento:
Article
/
Congress and conference