Effect of bacterial lipopolysaccharide on gastric emptying of liquids in rats
Braz. j. med. biol. res
; 30(2): 207-211, Feb. 1997. graf
Article
em En
| LILACS
| ID: lil-188428
Biblioteca responsável:
BR1.1
RESUMO
The objectives of the present investigation were 1) to study the effect of bacterial lipopolysaccharide (LPS) on rat gastric emptying (GE) and 2) to investigate a possible involvement of the vagus nerve in the gastric action of LPS. Endotoxin from E. coli (strain 055B5) was administered sc, ip or iv to male Wistar rats (220-280 g body weight) at a maximum dose of 50 mug/kg animal weight. Control animals received an equivalent volume of sterile saline solution. At a given time period after LPS administration, GE was evaluated by measuring gastric retention 10 min after the orogastric infusion of a test meal (2 ml/l00 g animal weight), which consisted of 0.9 per cent NaCl plus the marker phenol red (6 mg/dl). One group of animals was subjected to bilateral subdiaphragmatic vagotomy or sham operation 15 days before the test. A significant delay in GE of the test meal was observed 5 h after iv administration of the endotoxin at the dose of 50 mug/kg animal weight. The LPS-induced delay of GE was detected as early as 30 min and up to 8 h after endotoxin administration. The use of different doses of LPS ranging from 5 to 50 mug/kg animal weight showed that the alteration of GE was dose dependent. In addition, vagotomized animals receiving LPS displayed a GE that was not significantly different from that of the sham control group. However, a participation of the vagus nerve in LPS-induced delay in GE could not be clearly demonstrated by these experiments since vagotomy itself induced changes in this gastric parameter. The present study provides a suitable model for identifying the mechanisms underlying the effects of LPS on gastric emptying.
Texto completo:
1
Índice:
LILACS
Assunto principal:
Infecções Bacterianas
/
Vagotomia
/
Lipopolissacarídeos
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Escherichia coli
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Esvaziamento Gástrico
Limite:
Animals
Idioma:
En
Revista:
Braz. j. med. biol. res
Assunto da revista:
BIOLOGIA
/
MEDICINA
Ano de publicação:
1997
Tipo de documento:
Article